University of California San Diego
Location
La Jolla, CA | United States
Job description
UCSD Layoff from Career Appointment : Apply by 02/21/2024 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants : Apply by 03/01/2024. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The School of Biological Sciences is comprised of four academic departments, Cell and Developmental Biology, Ecology Behavior and Evolution, Molecular Biology, and Neurobiology, which reflect broad foci in faculty research and in the academic programs offered by the School. Each department is headed by a Chair who works individually to represent their department, and works collaboratively as a member of the School's Council of Chairs (CoC) on those school concerns which cross the boundaries of section governance. The School has over 100 faculty FTE and approximately 5000 undergraduate students in 8 majors, with annual enrollments of approximately 30,000.
Under general supervision of the PI and other senior researchers, the Staff Research Associate 2 will work independently to make design and construct jumbo (>200 kb) bacteriophage genomes as yeast artificial chromosomes (YACs) using synthetic and semi-synthetic DNA. They will then introduce those YACs into appropriate bacterial host cells so that the input DNA commandeers the bacterial machinery to produce the new phage varieties as coded by the genomes. The work will involve computational genome design, synthetic biology to assemble phage genomes, yeast genetics, and basic bacteriophage techniques. In particular, much of the work will involve yeast. This will include yeast strain genetic manipulation, yeast transformation of DNA and selection on the appropriate yeast cell plates, preparing yeast cell extracts for various biochemical analyses, immunofluorescence, live cell imaging and RNA analyses.
Other duties include: analyzing and interpreting DNA sequence and analyzing genetic screens. The Staff Research Associate 2 will participate in writing manuscripts, and attend and present research results at laboratory meetings.
Strong theoretical and practical background and knowledge in biochemistry, molecular biology and microbiology as is typically acquired by a Bachelor's degree and by relevant lab experience in any of the related fields of study.
Strong demonstrated knowledge of, and experience in, basic biochemistry, chemistry, and basic molecular biology methods including PCR, oligonucleotide primer design, DNA sequencing, plasmid preparations, restriction enzyme digestions, sterile techniques media preparation, and statistical analysis.
Strong demonstrated experience with general lab equipment including centrifuges, gel electrophoresis apparatus, power supplies, and spectrophotometers.
Extensive experience in the preparation of scientific reagents including common buffers and stock solutions.
Proficient with Apple and PC computers and associated word processing, spreadsheet, and database software with a willingness and ability to update and increase skills and knowledge as needed.
Proven knowledge of MSDS's (Material Data Safety Sheets) and the hazard communication and Injury and Illness and Prevention Program (IIPP). Knowledge of laboratory safety precautions including appropriate clothing, safe handling of chemicals, and the use of protective items.
Ability to assist in preparation of detailed and accurate scientific reports.
Skill to train others in various lab techniques and procedures (safety precautions, preparation of media, dishwashing, equipment usage. laboratory maintenance, etc.).
Experience and willingness to read scientific papers in order to stay abreast of developments within the field and relating to current experiments.
Excellent oral and written communication skills with the ability to present data and updates on a regular basis.
Excellent organization and record-keeping skills.
Demonstrated experience and proven ability to perform work with minimal supervision under the challenge and stress of frequent interruptions and frequently changing priorities and deadlines. Ability to handle multiple tasks simultaneously requiring detail and accuracy.
Excellent interpersonal skills, ability to work with a diverse group of people, and communicate clearly and effectively to promote positive relationships.
Proven ability to maintain confidentiality in matters related to work.
Must be able to work some evenings and weekends to meet project deadlines and process experiments.
About University of California San Diego
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.
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