University of California San Diego
Location
La Jolla, CA | United States
Job description
UCSD Layoff from Career Appointment : Apply by 02/29/2024 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants : Apply by 03/11/2024. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Moores Cancer Center (MCC) is one of just 53 NCI-designated Comprehensive Cancer Centers in the United States and the only one in San Diego County. As a consortium cancer center, it is a collaborative partnership between the UCSD: encompassing 28 departments, 6 schools (School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Public Health, Jacobs School of Engineering, School of Biological Sciences & the School of Physical Sciences), UCSD Health oncology hospitals and clinics; the basic and public health research and outreach of San Diego State University (SDSU), and the basic and translational research of the La Jolla Institute of Immunology (LJI). These various programs and units are all dedicated to fulfilling the Moores Cancer Center's mission of reducing cancer's burden. As such, it ranks among the top centers in the nation conducting the continuum of cancer research, providing advanced patient care, and serving the community through outreach and education programs. As a top-ranking, future-oriented organization, we offer challenging career opportunities in a fast-paced and innovative environment. Moores Cancer Center follows a progressive philosophy of career-path development for its employees including opportunities for cross-training, professional development, and progressive responsibility.
MCC's mission is to transform cancer care in our catchment area and beyond by driving exceptional scientific discoveries and innovations in prevention, detection, care, and training. MCC will make a global impact on improving health by reducing cancer burden through accelerated discovery and translation, compassionate and interdisciplinary care, education and community engagement, with the foundation of our core values - Excellence, Innovation and Risk-Taking, Collaboration, Diversity and Service.
Under supervision, assist with characterization of oncogenic transformation and preclinical studies. Assist with recombinant DNA work and genome engineering. Assist with various cell culture techniques such as performing flow cytometry analysis of cell surface protein expression, cell propagation, contaminant testing, and cryopreservation. Assist with mouse colony management and genotyping using established PCR techniques. Work with small animals and assist with procedures such as surgery, injections and monitoring pregnancies. Assist with immunohistochemistry of frozen and fixed tissues or cells. Assist with protein biochemistry techniques including protein quantification, SDS PAGE, immunoprecipitation, and Western blotting. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication. Other duties assigned as needed.
Theoretical knowledge of molecular biology, immunology, cell biology, and/or biochemistry.
Ability to perform molecular biology techniques including PCR, RT-PCR, cDNA synthesis, gene expression analysis, recombinant protein expression, RNA/DNA isolation, protein purification.
Ability to perform cell biology techniques including: mammalian cell culture, DNA transfection, cell fractionation and differential centrifugation, fluorescence- and luminescence-based bioassays.
Ability and willingness to handle and work with small animals. Experience to assist with mouse genotyping using DNA isolation, established PCR-based methods and argarose gel electrophoresis. Ability to assist in performing mouse surgeries, experiments, and injections.
Ability to perform biochemical assays including protein quantification using BCA, running SDS-PAGE gel, immunoblot and co-immunoprecipitation.
Ability to perform DNA and protein characterization techniques using electrophoretic analysis.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Ability to maintain confidentiality. Organizational skills to order and maintain lab equipment and supplies.
Basic understanding of computer applications such as Word, Excel and PowerPoint. Internet search and software analysis capabilities are preferred.
Detail-oriented. Ability to work with little supervision. Ability to follow verbal and/or written instructions. Ability to follow-up on previou
About University of California San Diego
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.
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