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Biological Scientist II - Gene Therapy Research


University of Florida


Location

Gainesville, FL | United States


Job description

Classification Title:

Biological Scientist II

Job Description:

The Department of Pediatrics, Child Health Research Institute, Dr. Byrne's Research Laboratory is accepting applications to fill the position of Biological Scientist II. This is a Biological Scientist position in a research lab studying adeno-associated virus (AAV) gene therapy. The Biological Scientist is expected to be able to 1. Develop/Edit protocols to improve existing techniques or to incorporate new diagnostic procedures; 2. Oversee their execution assigned experiments; 3. Review, approve, or interpret scientific results; 4. Conduct rodent procedures as necessary to complete research objectives 5. Present researching findings to PI and at laboratory meetings; 6. Maintain laboratory safety programs and train personnel in laboratory safety techniques; 7. Actively participate in the writing of grants, papers, and statements of work. 

The Biological Scientist II must be able to perform the following essential functions:

AAV Production:The primary responsibility of this position is to implement and optimize the production of rAAV in suspension using a methods currently established. The goal is to be able to reproduce the yields and quality of the vector produced in shaker flask up to 10L harvest scale. The incumbent will design protocols with the help of the supervisor, execute, analyze and report to the supervisor for review.

Animal Studies:Will participate in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v. injection.  Other duties with the mice may include doing echocardiography, sensory and motor function, force mechanics, and metabolic studies.  Collecting blood and tissues for processing in a clean and sterile manner. Individual will play a key role in regulatory management (IACUCs) and record keeping for each animal using a secure web database.

Molecular and Cellular Work:Testing of research, GLP and GMP-grade AAV and HSV vector preparations in compliance with cGMP for the qualitative and quantitative assessment. These activities require strict adherence to written protocols, proper reporting and data entry. During the course of these assays, the candidate will perform standard laboratory techniques that include, but are not limited to, cell culture, molecular biology (PCR, western blot, dot blot, hybridization, protein and nucleic acid electrophoresis), virological techniques (infection, transduction, viral gene expression and detection), microscopy, pH, conductivity. These activities included reagent preparations, storage and tracking of expiration date in compliance with GMP.

Standard Operating Procedures: Writing new and/or editing existing Standard Operating Procedures (SOPs), Test Records (TRs) and Quality Control Reagent Preparation records (QCRPs). 

Regulatory Compliance:Individual will ensure that laboratory is compliant with governing rules and regulations [IRB/IACUCU/Etc].  Coordinate all regulatory submissions/renewals.  Ensure all EH&S standards are met.  Train/ensure new laboratory members to ensure compliance standards are met.

OTHER: Perform tasks and experiments as directed by the Principal Investigator. Any other duties, as needed, to fulfill the mission and to abide by the values of the College of Medicine.

Expected Salary:

$45,600 - $50,100 annually; commensurate within this range based on qualifications and experience.

Minimum Requirements:

Master's degree in biology or a related field with 1 year of relevant experience, or an equivalent combination of education and experience. Bachelor's degree required.

Preferred Qualifications:

Special Instructions to Applicants:

To be considered for this position, please include a cover letter, resume, and a three professional references with your online application form.

 

Work schedule will be Monday through Friday, 8:00am to 5:00pm. Nights & weekends as needed for on-going research.

 

The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.

 

This is a time-limited position.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required:Yes


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