The John Innes Centre
Location
Norwich, Norfolk | United Kingdom
Job description
Hours per week 37 Project Title The role of RNA structures in plant response to stress Months Duration 36 Interview Date 30 Mar 2024
To study the role of RNA structure in the post-transcriptional regulation of gene expression in response to stress, mainly temperature. The successful candidate will join a team at JIC working on multiple levels of experiments: genome-wide in vivo RNA structure analysis targeted individual RNA functional motifs' characterisation and corresponding bioinformatics analysis.
The successful candidate will primarily interact with other researchers including biologists, chemists and computer scientists in the Ding Lab. They will also have regular interactions with the lab collaborators around the world. They will also interact with the other researchers across the Norwich Research Park.
Percentage Deep sequencing library construction and basic data analysis 50 in vivo RNA structure mapping 20 Biological function characterisation of individual RNA thermometer 15 Presentation of results as papers and seminars, participation in discussion with lab collaborators, attending meetings and seminars, outreach 10 Develop new methods for studying RNA structure 5 As agreed with the line manager, any other duties commensurate with the nature of the post, for example, contributing to the work of Institute committees
Requirement Importance Understanding of deep sequencing and RNA structure profiling technologies Essential PhD in Biology or Chemistry or Bioinformatics Essential
Requirement Importance Deep sequencing library construction and analysis Essential Molecular biology Essential RNA biology Essential Nucleic acid chemistry Essential Regulation of gene expression Essential RNA structure profiling background Essential
Requirement Importance Previous experience in genome-wide study Essential Previous experience of plant transformation Essential Previous experience of working on regulation of gene expression Essential Extensive experience in transcriptome study Desirable
Requirement Importance Evidence of intellectual independence, rigour and creativity Essential Demonstrated ability to work independently, using initiative and applying problem solving skills Essential Experience of oral presentation to scientific audiences Essential Good verbal and written communication skills Essential Good interpersonal skills with the ability to work well as part of a team and lead a team Essential
Requirement Importance Attention to detail Essential Willingness to embrace the expected values and behaviours of all staff at the Institute, ensuring it is a great place to work Essential Able to present a positive image of self and the Institute, promoting both the international reputation and public engagement aims of the Institute Essential Willingness to work outside standard working hours when required Essential Promotes equality and values diversity Essential
The John Innes Centre is an independent, international centre of excellence in plant and microbial genetics, carrying out fundamental and strategic research, to train scientists and making our findings available to society.
Our research makes use of a wide range of disciplines in the biological and chemical sciences including; cell biology, biochemistry, chemistry, genetics and molecular biology.
We pride ourselves on being a welcoming and inclusive working environment for all. We have a diverse and multicultural scientific community and thrive on our European and international links, appointing staff from across the world. Any candidate who would like further information on current or anticipated immigration requirements can contact the HR Team on +44 (0)1603 450888 or [email protected].
We are proud to hold a prestigious Athena SWAN Gold award , recognising the impact of our work in promoting gender equality in science. We are also a proud member of the Stonewall Diversity Champion’s programme , a founding signatory of the Tecnician Commitment and, as a Disability Confident employer, guarantee to offer an interview to all disabled applicants who meet the essential criteria for the post.
The John Innes Centre is a registered charity (No. 223852), limited by guarantee (registered in England No. 511709).
Cell & Developmental Biology
Development continues throughout the life of the plant. While many linear pathways affecting development have been discovered and are illustrated by elegant genetic studies, the challenge of the future will be for us to understand how these developmental pathways are integrated, both with themselves as well as with environmental signals, to enable a mature plant to grow from a seed. Guided by evolutionary principles, the Department uses its knowledge of biological processes obtained in laboratory organisms such as Arabidopsis to understand processes in crop plants such as Brassicas and cereals. To study this interaction of the plant genome with internal developmental networks and the environment, the Department uses a combination of genetic, cell biological, molecular and evolutionary strategies. The insights we are gaining provide both an increasingly detailed knowledge of the proteins involved in diverse regulatory mechanisms and a conceptual framework in which this knowledge can be related and eventually integrated into a molecular description of plant cell function. The knowledge the department is creating is relevant to agriculture. Genes that control plant stature, form, over-wintering responses and growth are potentially useful for improving crop performance.
The Ding lab investigates the role of RNA structure in the transcriptional and post-transcriptional regulation of gene expression, including translation, polyadenylation, RNA splicing, RNA stability, RNA export, and RNA processing. We are also exploring how RNA structure alters in response to environmental changes. We are developing new experimental and analytical approaches for revealing RNA structure and RNA-protein interactions. We developed two novel and powerful platforms to study RNA structure in vivo and across diverse species at both the genome-wide scale and in individual RNAs. These breakthroughs have removed technological barriers and opened up new avenues for RNA biology research. Our lab utilizes multidisciplinary knowledge and approaches in the areas of nucleic acid chemistry, RNA biology and bioinformatics.
Postdoctoral Researcher
Salary: £35,300 - £43,750 per annum depending on qualifications and experience.
Contract: 3 years, full time
Location: John Innes Centre, Norwich, UK.
Closing date: 20 March 2024
Reference: 1004625
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